阿反相离子对高效液相色谱法（RP - HPIPC）为一肝素衍生的寡糖复杂的混合物分离方法开发了一个流动相逐步优化，其中的离子浓度配对试剂，流动相pH值，乙腈浓度变化。 The resolution该决议
of more than 30 oligosaccharide components was obtained, under optimized conditions, in an analysis time of less than 30 min.超过30寡糖成分，得到优化的下不到30分钟的分析时间条件。 This represents the first RP-HPLC method that can separate a complex mixture of both small and large sulfated oligosaccharides in a single chromatographic step.这是首次反相高效液相色谱法，可以在一个单一的色谱分离步骤的小型和大型硫酸寡糖的复杂混合物。 The heparin-derived oligosaccharides, in this mixture, can also be肝素寡糖，在这种混合物，也可以
separated under a second set of RP-HPIPC conditions using a volatile ion-pairing reagent, tributylammonium acetate, to aid in the recovery of individual sulfated oligosaccharides.下一个反相HPIPC条件的第二套分开使用挥发性离子对试剂，tributylammonium醋酸，以协助个别硫酸寡糖复苏。 Moreover, it was possible to replace sodium chloride gradient,此外，有可能取代氯化钠梯度，
required for eluting highly sulfated oligosaccharides, with a fixed, low concentration of a volatile salt, ammonium acetate, by utilizing an acetonitrile gradient.需要洗脱高度硫酸寡糖，与一个固定的动荡盐，低浓度醋酸铵，利用一乙腈梯度。 This solvent system might make it possible to directly interface this RP-HPIPC separation with mass spectral analysis.这种溶剂系统，因此可能直接接口这个质谱分析反相HPIPC分离。
2003 Elsevier BV All rights reserved. ?2003埃尔塞维尔BV公司版权所有。Keywords: Ion-pairing reagents; Gradient elution; Oligosaccharides关键词：离子配对试剂;梯度洗脱;寡糖